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“Sticky Bone” Preparation Device: A Pilot Study on the Release of Cytokines and Growth Factors,Materials
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Sticky bone, a growth factor-enriched bone graft matrix, is a promising autologous material for bone tissue regeneration. However, its production is strongly dependent on manual handling steps. In this sense, a new device was developed to simplify the confection of the sticky bone, named Sticky Bone Preparation Device (SBPD®). The purpose of this pilot study was to investigate the suitability of the SBPD® to prepare biomaterials for bone regeneration with autologous platelet concentrates. The SBPD® allows the blending of particulate samples from synthetic, xenograft, or autogenous bone with autologous platelet concentrates, making it easy to use and avoiding the need of further manipulations for the combination of the materials. The protocol for the preparation of sticky bone samples using the SBPD® is described, and the resulting product is compared with hand-mixed SB preparations regarding in vitro parameters such as cell content and the ability to release growth factors and cytokines relevant to tissue regeneration. The entrapped cell content was estimated, and the ability to release biological mediators was assessed after 7 days of incubation in culture medium. Both preparations increased the leukocyte and platelet concentrations compared to whole-blood samples (p < 0.05), without significant differences between SB and SBPD®. SBPD® samples released several growth factors, including VEGF, FGFb, and PDGF, at concentrations physiologically equivalent to those released by SB preparations. Therefore, the use of SBPD® results in a similar product to the standard protocol, but with more straightforward and shorter preparation times and less manipulation. These preliminary results suggest this device as a suitable alternative for combining bone substitute materials with platelet concentrates for bone tissue regeneration.
中文翻译：
粘性骨是一种富含生长因子的骨移植基质，是一种很有前途的自体骨组织再生材料。然而，它的生产很大程度上依赖于手动处理步骤。从这个意义上说，开发了一种新设备来简化粘骨的制作过程，称为粘骨制备设备 (SBPD®)。这项试点研究的目的是研究 SBPD® 是否适合用自体血小板浓缩物制备用于骨再生的生物材料。SBPD® 允许将来自合成、异种移植或自体骨的颗粒样品与自体血小板浓缩物混合，使其易于使用，并且无需对材料组合进行进一步操作。描述了使用 SBPD® 制备粘性骨样品的方案，并将所得产品与手动混合的 SB 制剂进行比较，以了解细胞含量以及释放与组织再生相关的生长因子和细胞因子的能力等体外参数。估计包埋的细胞含量，并在培养基中孵育 7 天后评估释放生物介质的能力。与全血样品相比，两种制剂都增加了白细胞和血小板浓度（p < 0.05），SB 和 SBPD® 之间没有显着差异。SBPD® 样品释放了几种生长因子，包括 VEGF、FGFb 和 PDGF，其生理浓度与 SB 制剂释放的浓度相当。因此，使用 SBPD® 可得到与标准方案类似的产品，但制备时间更直接、更短，操作更少。 |
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